Top 10 B2B VC (Venture Capital) Funds in Czech Republic in May 2025

Last updated: May 2025 A list of 10 VC (Venture Capital) funds that invest in B2b startups based in Czech republic. We rank investors based on the number of investments they made in B2b companies from Czech republic. We update this investor list every month.

Top 10 B2B VC (Venture Capital) Funds in Czech Republic in May 2025

Investor	B2B Czech Republic investments
Jet Investment	1
Tilia Impact Ventures	1
J&T Ventures	1
LEVERAGE	1
SpeedUp Venture Capital Group	1
Rockaway Capital	1
Patero	1
Patero	1
PortfoLion Capital Partners	1
Presto Ventures	1

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Investor list

Where others see industry, we see investment opportunity. For 20 years in private equity, we have helped 25 industrial companies to breathe again. By managing, developing and selling, we pursue a simple goal - to achieve high returns without unnecessary risk. Qualified investors invest with us through Jet funds.

Investment focus

B2B, Industrial, Artificial Intelligence
Seed
Czech Republic

Portfolio highlights

PARTORY — PARTORY simplifies sourcing custom metal parts with 8,000+ machines across Europe. Manage orders and inquiries easily in our app.

Tilia Impact Ventures is a Czech impact fund that supports purpose-driven founders and companies with scalable business models and high environmental or social impact potential.

Investment focus

Information Technology, Health Care, Marketplace
Seed, Pre-Seed, Funding Round
Czech Republic, Estonia, Italy

Portfolio highlights

Alvie — Alvie creates products based on sensors and an AI platform to assist farmers in adjusting pesticide doses.
FaceUp — Whistleblowing System for companies and schools. FaceUp is an anonymous reporting channel for those who care. Try FaceUp for FREE.
LetsData — Early discovery and response coordination to media anomalies

J&T Ventures is an early-stage Prague-based Venture Capital fund, which invests in promising startups from the CEE and SEE region. Our strategy is industry agnostic, but we primarily focus on companies in the seed stage with proven product market fit. Our average ticket size is between EUR 0.5m - EUR 2.5m.

Investment focus

Software, Information Technology, Artificial Intelligence
Seed, Funding Round, Series A
Czech Republic, United States, Croatia

Portfolio highlights

XUND — Answer the most important health questions with AI-powered technology from XUND. Medically certified and CE marked.
Wultra — Wultra helps the leading banks and fintech companies to secure their digital systems and bring trust to the customer journeys. Our security-related software technologies cover the whole digital banking application stack, be it on the web or mobile platforms.
Grid.online — Grid.online is building transportation infrastructure as a service that will unlock the huge free transportation capacity of cities and allow it to be shared efficiently.

LEVERAGE build exceptional internet companies. We invest our own experience, time and capital. We are looking for new ambitious projects!

Investment focus

B2B, E-Commerce, Retail Technology
Seed
Czech Republic

Portfolio highlights

Nulisec — Nulisec is on a mission to make European manufacturers of Fast Moving Consumer Goods more accessible to the world. They are starting with Foods & Beverages because this industry produces the most waste and loss from the inefficient matching of supply and demand from manufacturers to distribution and retail. They provide the technologyinfrastructure and marketing reach to help emerging brands and other businesses to leverage the power of new technology to engage with their users and customers and operate in a more efficient way. Retailers get access to a whole new dimension of products, through a unified ordering system which streamlines purchasing processes.

We are a group of venture capital funds, investing in enterprises in an early stage of development. Areas of our interest include enterprises and entrepreneurs in Central and Eastern Europe, who want to conquer the global market by utilizing their self-developed solutions. Our cooperation with companies is based on a 3-8 years investment horizon.We're looking for innovations from areas such as: consumer internet, electromobility, energy, fintech, martech, adtech, medtech, IoT and hardware. We're interested in technologies like Machine Learning, Picture Recognition and Artificial Intelligence.

Investment focus

Software, SaaS, Artificial Intelligence
Seed, Pre-Seed, Series A
Poland, United States, Czech Republic

Portfolio highlights

PARTORY — PARTORY simplifies sourcing custom metal parts with 8,000+ machines across Europe. Manage orders and inquiries easily in our app.
CYCLE — GetHenry is a rapidly growing subscription service for market-leading delivery companies. We make sure that thousands of couriers are on the move safely, quickly and comfortably every day. In this way, we not only create added value for delivery services, but also help to reduce transport-related CO2 emissions on the last mile.
Patron GO — Patron GO is a fintech firm that uses AI to detect unnecessarily high costs on a customer's bank account and offers the best solutions.

We focus on investing and building Internet businesses not only in CEE. We believe in innovations and technologies. We create some added value. We are the next generation of entrepreneurs.

Investment focus

E-Commerce, Software, Fashion
Seed, Funding Round, Series A
Czech Republic, Germany, United States

Portfolio highlights

DoDo — DoDo is the smart delivery service specializing in last-mile and same-day delivery of goods to the end customer. DoDo is currently the technological leader with its data-driven solution and agile processes. We already managed to ensure long-term contracts and pilot projects with multiple high-profile retail and gastro customers ensuring us asubstantial turnover growth and margins. In DoDo we plan to invest in its SW system architecture to further optimize the delivery process based on big data, AI and automatization. The goal is to be the prime provider with its scalable solution. Same-day-delivery is expected to be an important service for all retail players and DoDo plans to continue with the acquisition of these clients especially in the Central European region. The indisputable advantage is easy implementation into the existing solution. Our flexible procedures and processes are easily adaptable to meet exactly what partner´s business needs. DoDo is managing over 600+ cars, 1300+ couriers in 4 countries.
Spotawheel — Spotawheel is a trusted high-tech used cars B2C platform that redesigns the whole buy-sell process from scratch, ensuring value, transparency and customer satisfaction at the highest levels throughout.
FreewayCamper — FreewayCamper offers the new digital platform with a large selection of top campers and additional services, with a direct booking option. They have the latest models to rent - fully equipped, fully covered, directly available, and easy to book. They give it at fair price guarantee & all kilometers included. They have short-term and long-termrentals and, it is flexible and offers to rebook.

Patero is an investment firm that invests in modern technologies. Patero is headquartered in Praha, Czech Republic.

Investment focus

Machine Learning, Software, Information Technology
Seed, Pre-Seed, Angel
Czech Republic

Portfolio highlights

Yedem — A reservation tool for corporate parking and employee carpooling. It allocates parking spaces and makes carpooling to work easier, saving money for everyone.
Outfindo — Making bikes & e-bikes buying easy
Flowlance — Flowlance help you automate your business with long-term clients

Private owned Investment company

Investment focus

B2B, SaaS, Retail
Pre-Seed
Czech Republic

Portfolio highlights

Yieldigo — Yieldigo is AI pricing platform that lets omnichannel retailers optimize their pricing strategies and predict KPIs. Learn more about how the new retail will look.

Portfolion is a CEE-focused multi-stage investor with a flexible fund structure that allows it to back companies from or before 1st client until the end of their journey. The fund manager has backed some of the CEE region’s most successful companies including SEON, Novakid, Tresorit, Packhelp, and Starschema.