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Top 10 B2B VC (Venture Capital) Funds in Czech Republic in May 2026

A list of 10 VC (Venture Capital) funds that invest in B2b startups based in Czech republic. We rank investors based on the number of investments they made in B2b companies from Czech republic. We update this investor list every month.

Top 10 B2B VC (Venture Capital) Funds in Czech Republic in May 2026

Investor B2B Czech Republic investments
Portfolion Capital Partners 1
J&T Ventures 1
Rockaway Capital 1
Jet Investment 1
Patero 1
LEVERAGE 1
Tilia Impact Ventures 1
SpeedUp Venture Capital Group 1
Patero 1
Presto Ventures 1

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Investor list
Portfolion Capital Partners
VC Fund · Budapest, Budapest, Hungary

Portfolion is a CEE-focused multi-stage investor with a flexible fund structure that allows it to back companies from or before 1st client until the end of their journey. The fund manager has backed some of the CEE region’s most successful companies including SEON, Novakid, Tresorit, Packhelp, and Starschema.

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Investment focus

  • Software, Information Technology, Big Data
  • Series A, Seed, Series B
  • Hungary, Poland, United States

Portfolio highlights

  • Novakid — Novakid - an online English school for children. Engaging and fun classes with native speakers for children 4-12 y.o.
  • Tree.ly — Tree.ly does climate protection through the projects in European forests through its SaaS platform and hyperlocal CO2-credits.
  • Mindgram — Mindgram is a science-based platform and mobile app for complete mental wellness.
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J&T Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

J&T Ventures is an early-stage Prague-based Venture Capital fund, which invests in promising startups from the CEE and SEE region. Our strategy is industry agnostic, but we primarily focus on companies in the seed stage with proven product market fit. Our average ticket size is between EUR 0.5m - EUR 2.5m.

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Investment focus

  • Software, Information Technology, Artificial Intelligence
  • Seed, Funding Round, Series A
  • Czech Republic, United States, Croatia

Portfolio highlights

  • XUND — Answer the most important health questions with AI-powered technology from XUND. Medically certified and CE marked.
  • Wultra — Wultra helps the leading banks and fintech companies to secure their digital systems and bring trust to the customer journeys. Our security-related software technologies cover the whole digital banking application stack, be it on the web or mobile platforms.
  • Grid.online — Grid.online is building transportation infrastructure as a service that will unlock the huge free transportation capacity of cities and allow it to be shared efficiently.
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Rockaway Capital
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

We focus on investing and building Internet businesses not only in CEE. We believe in innovations and technologies. We create some added value. We are the next generation of entrepreneurs.

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Investment focus

  • E-Commerce, Software, Fashion
  • Seed, Funding Round, Series A
  • Czech Republic, Germany, United States

Portfolio highlights

  • DoDo — DoDo is the smart delivery service specializing in last-mile and same-day delivery of goods to the end customer. DoDo is currently the technological leader with its data-driven solution and agile processes. We already managed to ensure long-term contracts and pilot projects with multiple high-profile retail and gastro customers ensuring us asubstantial turnover growth and margins. In DoDo we plan to invest in its SW system architecture to further optimize the delivery process based on big data, AI and automatization. The goal is to be the prime provider with its scalable solution. Same-day-delivery is expected to be an important service for all retail players and DoDo plans to continue with the acquisition of these clients especially in the Central European region. The indisputable advantage is easy implementation into the existing solution. Our flexible procedures and processes are easily adaptable to meet exactly what partner´s business needs. DoDo is managing over 600+ cars, 1300+ couriers in 4 countries.
  • Spotawheel — Spotawheel is a trusted high-tech used cars B2C platform that redesigns the whole buy-sell process from scratch, ensuring value, transparency and customer satisfaction at the highest levels throughout.
  • FreewayCamper — FreewayCamper offers the new digital platform with a large selection of top campers and additional services, with a direct booking option. They have the latest models to rent - fully equipped, fully covered, directly available, and easy to book. They give it at fair price guarantee & all kilometers included. They have short-term and long-termrentals and, it is flexible and offers to rebook.
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Jet Investment
VC Fund · Brno, Jihomoravsky kraj, Czech Republic · 1 investment in the past 12 months

Where others see industry, we see investment opportunity. For 20 years in private equity, we have helped 25 industrial companies to breathe again. By managing, developing and selling, we pursue a simple goal - to achieve high returns without unnecessary risk. Qualified investors invest with us through Jet funds.

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Investment focus

  • B2B, Industrial, Artificial Intelligence
  • Seed
  • Czech Republic

Portfolio highlights

  • PARTORY — PARTORY offers complete manufacturing solutions in engineering. PARTORY utilising AI and machine learning to streamline the procurement process for low-cost metal parts
  • International Automotive Group
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Patero
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

Private owned Investment company

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Investment focus

  • B2B, SaaS, Retail
  • Pre-Seed
  • Czech Republic

Portfolio highlights

  • Yieldigo — Yieldigo is AI pricing platform that lets omnichannel retailers optimize their pricing strategies and predict KPIs. Learn more about how the new retail will look.
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LEVERAGE
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

LEVERAGE build exceptional internet companies. We invest our own experience, time and capital. We are looking for new ambitious projects!

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Investment focus

  • B2B, E-Commerce, Retail Technology
  • Seed
  • Czech Republic

Portfolio highlights

  • Nulisec — Nulisec is on a mission to make European manufacturers of Fast Moving Consumer Goods more accessible to the world. They are starting with Foods & Beverages because this industry produces the most waste and loss from the inefficient matching of supply and demand from manufacturers to distribution and retail. They provide the technologyinfrastructure and marketing reach to help emerging brands and other businesses to leverage the power of new technology to engage with their users and customers and operate in a more efficient way. Retailers get access to a whole new dimension of products, through a unified ordering system which streamlines purchasing processes.
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Tilia Impact Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

Tilia Impact Ventures is a Czech impact fund that supports purpose-driven founders and companies with scalable business models and high environmental or social impact potential.

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Investment focus

  • Information Technology, Software, Health Care
  • Seed, Pre-Seed, Funding Round
  • Czech Republic, Estonia, Italy

Portfolio highlights

  • LetsData — LetsData is an AI-power radar against disinformation. LetsData provides real-time media and social media monitoring in over 50 countries, empowering state agencies, businesses, and civil societies to proactively navigate risks and seize opportunities in the face of disinformation.
  • Lifeyear — Digital-first heart care. At scale.
  • Theorema — Autonomous R&D agents for biotech, pharma, chemicals, and materials. Theorema analyzes data, recovers R&D costs, and accelerates discovery. #1 on BixBench.
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SpeedUp Venture Capital Group
VC Fund · Poznan, Wielkopolskie, Poland

We are a group of venture capital funds, investing in enterprises in an early stage of development. Areas of our interest include enterprises and entrepreneurs in Central and Eastern Europe, who want to conquer the global market by utilizing their self-developed solutions. Our cooperation with companies is based on a 3-8 years investment horizon.We're looking for innovations from areas such as: consumer internet, electromobility, energy, fintech, martech, adtech, medtech, IoT and hardware. We're interested in technologies like Machine Learning, Picture Recognition and Artificial Intelligence.

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Investment focus

  • Software, SaaS, Artificial Intelligence
  • Seed, Pre-Seed, Series A
  • Poland, United States, Czech Republic

Portfolio highlights

  • PARTORY — PARTORY offers complete manufacturing solutions in engineering. PARTORY utilising AI and machine learning to streamline the procurement process for low-cost metal parts
  • CYCLE — GetHenry is a rapidly growing subscription service for market-leading delivery companies. We make sure that thousands of couriers are on the move safely, quickly and comfortably every day. In this way, we not only create added value for delivery services, but also help to reduce transport-related CO2 emissions on the last mile.
  • Patron GO — Patron GO is a fintech firm that uses AI to detect unnecessarily high costs on a customer's bank account and offers the best solutions.
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Patero
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

Patero is an investment firm that invests in modern technologies. Patero is headquartered in Praha, Czech Republic.

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Investment focus

  • Software, Machine Learning, SaaS
  • Seed, Pre-Seed, Angel
  • Czech Republic

Portfolio highlights

  • Yedem — We eliminate problems associated with with overcrowded parking lots, lack of available parking in cities and high car pollution. Our goal is to make commuting efficient, convenient and sustainable. Striving for a happier and greener life for us all.
  • Flowlance — Flowlance help you automate your business with long-term clients
  • Boost.space — Combining the power of a Cloud-based Database with an intuitive Integration Platform, we make it easy to streamline and enhance your workflows. Imagine the simplicity of Airtable fused with the connectivity of Zapier. Experience the future of no-code Data Management, today.
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Presto Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic

We're a Prague-based VC firm investing in fast growing tech companies and empowering talented startup founders from Central and Eastern Europe.

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Investment focus

  • SaaS, Software, Information Technology
  • Seed, Pre-Seed, Series A
  • Czech Republic, United States, Estonia

Portfolio highlights

  • Corn — Corn is a new Ethereum Layer 2 network that aims to be the best platform for utilizing Bitcoin.
  • PilotGo — Developer platform for secure data sharing with multiple partners powered by Digital Twins.
  • The New Era® of Shopping — The New Era® of Shopping helps merchants make their catalogues discoverable and shoppable inside AI assistants. Key features of the platform include two-way catalog syncing with PIMs/ERPs, SKU-level intelligence to measure AI visibility and ranking of products inside LLM responses, user prompts analysis for trends and volume insights, andautomated content optimization to improve how products rank in conversational answers. The product is already integrated with all major eCommerce platforms like Shopify, WooCommerce, Magento, BigCommerce, Wix, etc, as well as main AI discovery platforms: ChatGPT, Gemini, Claude, Google AI Mode, and Perplexity.
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