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Top 10 Medical Startup Investors in Czech Republic (June 2026)

The most active venture capital funds and angel investors investing in Czech Republic Medical startups. This investor list is updated monthly from the Shizune investor database, ranked by number of investments in Czech Republic Medical startup companies. Find investors to raise a Pre-Seed, Seed or Series A round with Shizune.
Investor Relevant Deals Geography Stage Focus Round Size
Purple Ventures
VC Fund
5 Czech Republic, United Kingdom, India Seed, Pre-Seed Software, Health Care, Medical $132k–$13M
MITON
VC Fund
4 Czech Republic, United Kingdom, Poland Seed, Pre-Seed Software, E-Commerce, Internet $219k–$4.5M
Reflex Capital
VC Fund
3 Czech Republic, United States, United Kingdom Seed, Series A, Pre-Seed Software, Information Technology, SaaS $250k–$10M
N1
VC Fund
3 Czech Republic, Germany, United States Seed, Pre-Seed Software, Information Technology, Health Care $120k–$3.1M
RSJ Investments SICAV a.s.
VC Fund
3 Germany, Czech Republic, Slovakia (Slovak Republic) Seed, Series A Artificial Intelligence, Robotics, Software $1.3M–$11M
i&i Biotech Fund
VC Fund
3 Germany, Czech Republic, United Kingdom Seed, Series A, Pre-Seed Biotechnology, Medical, Health Care $369k–$20M
Martin Rozhon
Angel
2 Czech Republic, Germany Pre-Seed, Seed, Angel Software, E-Commerce, Health Care $235k–$478k
KHAN Technology Transfer Fund
VC Fund
2 Germany, Austria, Luxembourg Seed, Series A, Venture - Series Unknown Biotechnology, Medical, Biopharma $259k–$11M
Pilulka Lab
VC Fund
1 Czech Republic Pre-Seed Medical, Software, Health Care
AspectWorks
VC Fund
1 Czech Republic Seed Apps, Medical, Software
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Investor list

Purple Ventures
VC Fund · Brno, Jihomoravsky kraj, Czech Republic · 5 investments in Czech Republic Medical startups

We invest smart, kind money into early-stage purpose-driven technology startups with initial revenue and global ambitions.

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Investment focus

  • Industry: Software, Health Care, Medical
  • Stage:Seed, Pre-Seed
  • Geography: Czech Republic, United Kingdom, India

Investor stats

  • Total investments: 40
  • Round size: Seed $745k–$2.4M; Pre-Seed $132k–$13M
  • Lead/follow: 43% lead, 57% follow
  • Last fund: Likely still investing — fund raised in 2024

Portfolio highlights

  • Tuito — Tuito give e-commerce shoppers straightforward insurance with a tempting offer. We collaborate with digital merchants to provide them with cross-selling solutions that also increase customer pleasure and loyalty.
  • Sloneek — Sloneek is a cloud-based HR system that is easy to implement in small and medium companies. Your employees will love it & you will be able to manage your people more efficiently.
  • Flowlance — Flowlance help you automate your business with long-term clients
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MITON
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 4 investments in Czech Republic Medical startups

Company builders operating from Czech Republic and focusing on Central and Eastern Europe. We prefer building consumer facing businesses and have the most fun around marketplaces and e-commerce in general.

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Investment focus

  • Industry: Software, E-Commerce, Internet
  • Stage:Seed, Pre-Seed
  • Geography: Czech Republic, United Kingdom, Poland

Investor stats

  • Total investments: 58
  • Round size: Seed $300k–$4.5M; Pre-Seed $219k–$1.1M
  • Lead/follow: 53% lead, 47% follow

Portfolio highlights

  • PangeAI
  • Bandits — Bandits is well-positioned within this broader framework of AI-enabled business transformation.
  • Kolay Randevu — Kolay Randevu is an online beauty booking marketplace and salon management software for salons.
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Reflex Capital
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 3 investments in Czech Republic Medical startups

Doing business may resemble a jungle survival. You better be prepared and have the right reflexes! It's also useful to have someone with real experience around. We at Reflex Capital are entrepreneurs, just like you. We built our own companies, negotiated with investors, and took part in plenty of transactions.

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Investment focus

  • Industry: Software, Information Technology, SaaS
  • Stage:Seed, Series A, Pre-Seed
  • Geography: Czech Republic, United States, United Kingdom

Investor stats

  • Total investments: 78
  • Round size: Seed $250k–$3M; Pre-Seed $750k–$1.2M; Series A $2M–$10M
  • Lead/follow: 29% lead, 71% follow
  • Last fund: May invest from a new fund soon — raised $10M in 2016

Portfolio highlights

  • FaceUp — Whistleblowing System for companies and schools. FaceUp is an anonymous reporting channel for those who care. Try FaceUp for FREE.
  • Fitify — Your personal workout app. Workout routines & training plan at home.
  • Flow Nutrition — Flow develops products for the proper functioning of the body in the demanding conditions of the 21st century
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N1
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 3 investments in Czech Republic Medical startups

NATION 1 IS “DAY 0” INVESTOR, THE 1ST CHOICE FOR THE 1ST INVESTOR & PARTNER FOR BUILDING A SUCCESSFUL COMPANY.

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Investment focus

  • Industry: Software, Information Technology, Health Care
  • Stage:Seed, Pre-Seed
  • Geography: Czech Republic, Germany, United States

Investor stats

  • Total investments: 66
  • Round size: Seed $120k–$3.1M; Pre-Seed $235k–$2M
  • Lead/follow: 30% lead, 70% follow
  • Last fund: May invest from a new fund soon — last raise in 2019

Portfolio highlights

  • Bevy — Bevy is a delivery services that utilizes electric cars to transport parcels and couriers.
  • GoodAccess — Secure & Simplify your Team's access to cloud resources and systems. Secure Access Service Edge (SASE). Dedicated gateway. Access control. Try it Free.
  • DOT Glasses — Get high-quality, affordable prescription glasses from Dot Glasses. Stylish, durable, and budget-friendly eyewear for clear vision. Buy Now, Wear Now!
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RSJ Investments SICAV a.s.
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 3 investments in Czech Republic Medical startups

RSJ Private Equity manages a broad investment portfolio in real estate, energy industry, agriculture, biotechnologies and other economic sectors. We focus on key industries for the 21st century, such as power generation from renewables, sustainable and healthy food production, information technology, and life sciences, applied research leading to alonger and better human life. In addition to considering profitability, we take into account our projects´ potential to contribute to significant shifts in the given industry or field of activity.

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Investment focus

  • Industry: Artificial Intelligence, Robotics, Software
  • Stage:Seed, Series A
  • Geography: Germany, Czech Republic, Slovakia (Slovak Republic)

Investor stats

  • Total investments: 12
  • Round size: Seed $1.3M–$11M; Series A $1.4M–$9.9M
  • Lead/follow: 8% lead, 92% follow

Portfolio highlights

  • Hedepy — Online therapy works. Research has proven that what matters is the conversation, not where the conversation happens.
  • Neotiv — Enabling cognitive health
  • Brightpick — Brightpick offers AI robots for warehouses to easily automate every step of their order fulfillment. The innovative Brightpick robots enable warehouses of any size to fully automate order picking, consolidation and dispatch, as well as stock replenishment. Brightpick Autopicker, the company’s award-winning flagship robot, is the only mobile robotin the world that robotically picks and consolidates orders directly in warehouse aisles. The Brightpick solution takes just weeks to deploy and allows companies to reduce their fulfillment labor to a minimum. Headquartered near Cincinnati, OH, Brightpick has more than 200 employees and hundreds of AI robots deployed across the US and Europe.
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i&i Biotech Fund
VC Fund · Luxembourg, Luxembourg, Luxembourg · 3 investments in Czech Republic Medical startups

We are i&i Biotech Fund, partners of outstanding founders on their path to global success. We invest in innovative early stage Life Sciences startups.

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Investment focus

  • Industry: Biotechnology, Medical, Health Care
  • Stage:Seed, Series A, Pre-Seed
  • Geography: Germany, Czech Republic, United Kingdom

Investor stats

  • Total investments: 16
  • Round size: Seed $369k–$9.3M; Series A $5.8M–$20M
  • Lead/follow: 31% lead, 69% follow

Portfolio highlights

  • Theorema — Autonomous R&D agents for biotech, pharma, chemicals, and materials. Theorema analyzes data, recovers R&D costs, and accelerates discovery. #1 on BixBench.
  • Captain T Cell — Captain T Cell is a start-up project that develops T cell-based immunotherapies for cancer patients. The company identifies novel high-efficiency TCRs for the development of T cell immunotherapies. It focuses on CMC- and preclinical development for their lead candidates.
  • Ternary Therapeutics — Ternary Therapeutics is a biotechnology research center that specializes in bioinformatics and medicinal chemistry services.
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Martin Rozhon
Angel · Chrudim, Pardubicky kraj, Czech Republic · 2 investments in Czech Republic Medical startups

Martin Rozhon

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Investment focus

  • Industry: Software, E-Commerce, Health Care
  • Stage:Pre-Seed, Seed, Angel
  • Geography: Czech Republic, Germany

Investor stats

  • Total investments: 10
  • Round size: Pre-Seed $235k–$478k
  • Lead/follow: 30% lead, 70% follow

Portfolio highlights

  • Fabini — Fabini is a manufacturing and retail company that offers cookware products.
  • snuggs — In Europe alone, there are over 300 million people with a vagina. Half of them are of reproductive age and they menstruate. For many, it is often connected with struggle, stress, and discomfort. And they seek a change.
  • Puravia — Puravia provides food supplements. They also provide omega-3 fatty acids, multivitamins, minerals, proteins, digestive enzymes, cannabinoids, antioxidants, medicinal mushrooms, Probiotics, prebiotics, herbal food supplements, and glutathione.
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KHAN Technology Transfer Fund
VC Fund · Dortmund, Nordrhein-Westfalen, Germany · 2 investments in Czech Republic Medical startups

KHAN Technology Transfer Fund operates as an investment firm.

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Investment focus

  • Industry: Biotechnology, Medical, Biopharma
  • Stage:Seed, Series A, Venture - Series Unknown
  • Geography: Germany, Austria, Luxembourg

Investor stats

  • Total investments: 11
  • Round size: Seed $259k–$10M; Series A $5.5M–$11M
  • Lead/follow: 45% lead, 55% follow

Portfolio highlights

  • Casinvent Pharma — Casinvent Pharma develops new therapeutic options for the treatment of selected malignancies.
  • iDEL Therapeutics — iDEL Therapeutics is advancing a new class of cancer medicines by delivering therapeutic payloads directly into the cytosol of tumor cells through a unique, pan-cancer uptake mechanism.
  • KyDo Therapeutics — KyDo Therapeutics is a biotechnology company that develops novel covalent-allosteric small molecule inhibitors.
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Pilulka Lab
VC Fund · 1 investments in Czech Republic Medical startups

Pilulka Lab is a business acceleration and ventures funding company focused on investing in startups operating in the e-health industry.

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Investment focus

  • Industry: Medical, Software, Health Care
  • Stage:Pre-Seed
  • Geography: Czech Republic

Investor stats

  • Total investments: 2
  • Lead/follow: 50% lead, 50% follow

Portfolio highlights

  • Vitadio — The first digital therapy for type 2 diabetes covered by all statutory health insurers in Germany.
  • Carebot — Carebot is a clinically validated and certified AI solution that enables faster and more accurate interpretation of medical imaging. It automatically detects suspicious findings and supports early detection of serious oncological and other diseases.
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AspectWorks
VC Fund · Chodov, Karlovarsky kraj, Czech Republic · 1 investments in Czech Republic Medical startups

AspectWorks develops tailored business applications, mainly on Java and JEE platforms. Customers appreciate their quality and the fact that they keep their deadlines and meet agreed requirements.

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Investment focus

  • Industry: Apps, Medical, Software
  • Stage:Seed
  • Geography: Czech Republic

Investor stats

  • Total investments: 1
  • Lead/follow: 100% lead, 0% follow

Portfolio highlights

  • Hedepy — Online therapy works. Research has proven that what matters is the conversation, not where the conversation happens.
Show all investments...

Medical Investor funding in Czech Republic at a glance

Stats across 20 deals from 24 investors.

24
investors
in Czech Republic Medical startups
20
deals
in Czech Republic Medical startups
$1.3M
median round
in Czech Republic Medical startups
58%
lead investors
lead at least one round
45%
Seed
most common funding stage
88/13
VC / Angel
share of investor count

Access all 24 investors in Czech Republic Medical startups.

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