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Top 10 Biotech Startup Investors in Czech Republic (September 2026)

The most active venture capital funds and angel investors investing in Czech Republic Biotech startups. This investor list is updated monthly from the Shizune investor database, ranked by number of investments in Czech Republic Biotech startup companies. Find investors to raise a Pre-Seed, Seed or Series A round with Shizune.
Investor Relevant Deals Geography Stage Focus Round Size
J&T Ventures
VC Fund
2 Czech Republic, United States, United Kingdom Seed, Series A, Pre-Seed Software, Information Technology, Artificial Intelligence $317k–$14M
Presto Ventures
VC Fund
2 Czech Republic, United States, United Kingdom Seed, Pre-Seed, Series A Software, SaaS, Artificial Intelligence $132k–$32M
Vaclav Dejcmar
Angel
2 United States, Czech Republic Angel, Pre-Seed Video, Events, Software
Garage Angels
VC Fund
2 Czech Republic, Austria Seed, Venture - Series Unknown, Pre-Seed Software, Medical, Sensor $444k–$1.7M
JIC Ventures
VC Fund
2 Czech Republic Seed, Pre-Seed, Venture - Series Unknown Software, Medical, Information Technology $23k–$1.9M
Airbridge Equity Partners
VC Fund
1 The Netherlands, Germany, United States Series A, Seed, Venture - Series Unknown Software, Information Technology, SaaS $588k–$14M
i&i Biotech Fund
VC Fund
1 Germany, Czech Republic, United Kingdom Seed, Series A, Pre-Seed Biotechnology, Medical, Health Care $369k–$20M
Charles University Innovations Prague
VC Fund
1 Czech Republic Seed Life Science, Medical Device, Nanotechnology
Nikola Pantovic
Angel
1 Czech Republic Pre-Seed Software, Neuroscience, Market Research
KOOPEO Ventures
VC Fund
1 Czech Republic, United Kingdom Seed, Pre-Seed, Venture - Series Unknown Software, Content, E-Commerce $444k–$1.4M
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Investor list

J&T Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 2 investments in Czech Republic Biotech startups, 9 in the past 12 months

J&T Ventures is an early-stage Prague-based Venture Capital fund, which invests in promising startups from the CEE and SEE region. Our strategy is industry agnostic, but we primarily focus on companies in the seed stage with proven product market fit. Our average ticket size is between EUR 0.5m - EUR 2.5m.

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Investment focus

  • Industry: Software, Information Technology, Artificial Intelligence
  • Stage:Seed, Series A, Pre-Seed
  • Geography: Czech Republic, United States, United Kingdom

Investor stats

  • Total investments: 77
  • Round size: Seed $669k–$3.4M; Pre-Seed $317k–$14M; Series A $1M–$7.8M
  • Lead/follow: 53% lead, 47% follow
  • Last fund: May invest from a new fund soon — raised $53M in 2019

Portfolio highlights

  • Wultra — Wultra helps the leading banks and fintech companies to secure their digital systems and bring trust to the customer journeys. Our security-related software technologies cover the whole digital banking application stack, be it on the web or mobile platforms.
  • Definic — 66% of IT projects fail. Not because of technology, but because you picked the wrong vendor. Definic replaces guesswork and fragmented workflows with one connected platform, from defining the problem to managing the right vendors at scale.
  • Grid.online — Grid.online is building transportation infrastructure as a service that will unlock the huge free transportation capacity of cities and allow it to be shared efficiently.
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Presto Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 2 investments in Czech Republic Biotech startups

We're a Prague-based VC firm investing in fast growing tech companies and empowering talented startup founders from Central and Eastern Europe.

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Investment focus

  • Industry: Software, SaaS, Artificial Intelligence
  • Stage:Seed, Pre-Seed, Series A
  • Geography: Czech Republic, United States, United Kingdom

Investor stats

  • Total investments: 115
  • Round size: Seed $646k–$3M; Pre-Seed $132k–$1.7M; Series A $1M–$32M
  • Lead/follow: 47% lead, 53% follow
  • Last fund: Likely still investing — raised $160M in 2024

Portfolio highlights

  • Occam Industries — Modernising automation for modern conflict environments. Sole European supplier to the Ukrainian front line.
  • SatVu — SatVu captures high resolution thermal data from space for a safer and more sustainable Earth.
  • The New Era® of Shopping — The New Era® of Shopping helps merchants make their catalogues discoverable and shoppable inside AI assistants. Key features of the platform include two-way catalog syncing with PIMs/ERPs, SKU-level intelligence to measure AI visibility and ranking of products inside LLM responses, user prompts analysis for trends and volume insights, andautomated content optimization to improve how products rank in conversational answers. The product is already integrated with all major eCommerce platforms like Shopify, WooCommerce, Magento, BigCommerce, Wix, etc, as well as main AI discovery platforms: ChatGPT, Gemini, Claude, Google AI Mode, and Perplexity.
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Vaclav Dejcmar
Angel · 2 investments in Czech Republic Biotech startups

Vaclav Dejcmar

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Investment focus

  • Industry: Video, Events, Software
  • Stage:Angel, Pre-Seed
  • Geography: United States, Czech Republic

Investor stats

  • Total investments: 3
  • Lead/follow: 67% lead, 33% follow

Portfolio highlights

  • SlidesLive — Event-Aufzeichnungen und Livestreaming
  • Behavio — Secure you marketing success with top behavioral market research tools for a fraction of the traditional price. Start tracking your brand and testing your ads.
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Garage Angels
VC Fund · Moravany, Jihomoravsky kraj, Czech Republic · 2 investments in Czech Republic Biotech startups

Garage Angels is an investment firm that invests in companies at the angel investment stage.

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Investment focus

  • Industry: Software, Medical, Sensor
  • Stage:Seed, Venture - Series Unknown, Pre-Seed
  • Geography: Czech Republic, Austria

Investor stats

  • Total investments: 17
  • Round size: Seed $579k–$1.7M; Pre-Seed $444k–$513k; Venture - Series Unknown $1.4M–$1.7M
  • Lead/follow: 18% lead, 82% follow

Portfolio highlights

  • Sendance — Sendance develops and sells electronics and sensors on permeable, stretchable, and structurally adapted substrates. Permeability and structural adaptation enable the integration of sensors in areas where this was previously not possible.
  • HTGmedical — Building medical technologies to make the world a better place.
  • Carebot — Carebot is a clinically validated and certified AI solution that enables faster and more accurate interpretation of medical imaging. It automatically detects suspicious findings and supports early detection of serious oncological and other diseases.
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JIC Ventures
VC Fund · Brno, Jihomoravsky kraj, Czech Republic · 2 investments in Czech Republic Biotech startups

JIC Ventures is an investment company which providing venture capital investments to graduates of its parent company's JIC programs.

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Investment focus

  • Industry: Software, Medical, Information Technology
  • Stage:Seed, Pre-Seed, Venture - Series Unknown
  • Geography: Czech Republic

Investor stats

  • Total investments: 23
  • Round size: Seed $23k–$1.9M; Pre-Seed $245k–$513k; Venture - Series Unknown $80k–$1.7M
  • Lead/follow: 30% lead, 70% follow

Portfolio highlights

  • HTGmedical — Building medical technologies to make the world a better place.
  • Carebot — Carebot is a clinically validated and certified AI solution that enables faster and more accurate interpretation of medical imaging. It automatically detects suspicious findings and supports early detection of serious oncological and other diseases.
  • GINA Software —
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Airbridge Equity Partners
VC Fund · Amsterdam, Noord-Holland, The Netherlands · 1 investments in Czech Republic Biotech startups

Amsterdam-based VC firm backing exceptional European founders building category-defining B2B software companies.

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Investment focus

  • Industry: Software, Information Technology, SaaS
  • Stage:Series A, Seed, Venture - Series Unknown
  • Geography: The Netherlands, Germany, United States

Investor stats

  • Total investments: 30
  • Round size: Seed $588k–$2.7M; Series A $2.4M–$14M; Venture - Series Unknown $1.2M–$2.7M
  • Lead/follow: 77% lead, 23% follow
  • Last fund: Likely still investing — raised $67M in 2023

Portfolio highlights

  • Artwod — ArtWod is an online art school and mobile drawing app designed to help artists of all levels learn to draw through structured, daily practice.
  • Sipfront — Sipfront helps telecom operators to deliver better services by automating 24/7 active monitoring and by providing a test framework to simulate customer calls at scale
  • Celebratix — Celebratix is a blockchain-based ticketing firm. It provides a secure and simple ticketing system based on blockchain technology, so consumers do not need to deal with bitcoin.
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i&i Biotech Fund
VC Fund · Luxembourg, Luxembourg, Luxembourg · 1 investments in Czech Republic Biotech startups

We are i&i Biotech Fund, partners of outstanding founders on their path to global success. We invest in innovative early stage Life Sciences startups.

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Investment focus

  • Industry: Biotechnology, Medical, Health Care
  • Stage:Seed, Series A, Pre-Seed
  • Geography: Germany, Czech Republic, United Kingdom

Investor stats

  • Total investments: 16
  • Round size: Seed $369k–$5.3M; Series A $5.8M–$20M
  • Lead/follow: 31% lead, 69% follow

Portfolio highlights

  • Antiverse — Designing biologics for 100 challenging targets
  • Theorema — We build causal disease models to help pharmaceutical and biotech companies find promising drug targets and new indications for existing assets.
  • Ternary Therapeutics — Ternary Therapeutics is a biotechnology research center that specializes in bioinformatics and medicinal chemistry services.
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Charles University Innovations Prague
VC Fund · Praha, Hlavni mesto Praha, Czech Republic · 1 investments in Czech Republic Biotech startups

Charles University Innovations Prague specializes in offering consulting and managerial services for projects with commercial potential.

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Investment focus

  • Industry: Life Science, Medical Device, Nanotechnology
  • Stage:Seed
  • Geography: Czech Republic

Investor stats

  • Total investments: 1
  • Lead/follow: 0% lead, 100% follow

Portfolio highlights

  • LAM-X — Discover LAM-X's patented light-activated technology. We design innovative antimicrobial nanofiber materials to purify water, air, and enable life-science discoveries.
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Nikola Pantovic
Angel · 1 investments in Czech Republic Biotech startups

Investment focus

  • Industry: Software, Neuroscience, Market Research
  • Stage:Pre-Seed
  • Geography: Czech Republic

Investor stats

  • Total investments: 1
  • Lead/follow: 0% lead, 100% follow

Portfolio highlights

  • Behavio — Secure you marketing success with top behavioral market research tools for a fraction of the traditional price. Start tracking your brand and testing your ads.
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KOOPEO Ventures
VC Fund · Prague, Hlavni mesto Praha, Czech Republic · 1 investments in Czech Republic Biotech startups

KOOPEO Ventures is an investment company focused on pre-seed and seed stage investments within CEE region. We are looking for opportunities to invest especially in emerging projects with potential of high growth of revenues and company value. We only invests our own money which allows us to make investment decisions very quickly.We typicallyinvest up to 500 000EUR especially in the areas of E-commerce, Mobile, Internet, Affiliate marketing and Fintech. Except of financial resources ,we offer for all of our startups, individual approach, support during establishment of business strategy and help with international expansion. We also provide access to our network of advisors, partners and contacts on domestic and foreign markets.

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Investment focus

  • Industry: Software, Content, E-Commerce
  • Stage:Seed, Pre-Seed, Venture - Series Unknown
  • Geography: Czech Republic, United Kingdom

Investor stats

  • Total investments: 13
  • Round size: Seed $1M–$1.4M; Pre-Seed $444k–$638k
  • Lead/follow: 46% lead, 54% follow

Portfolio highlights

  • TRIFFT Loyalty Platform — Build lasting customer relationships with TRIFFT's powerful loyalty platform. Drive engagement and boost retention with customizable programs. Learn more!
  • Kardi Ai — Kardi Ai is a healthtech startup focused on long-term heart monitoring. With its smart wearable and AI-powered app, it provides a 6× higher chance of detecting heart rhythm disorders than current methods, covering 7 types of arrhythmias. Backed by MDR class IIa medical certification, it empowers people to protect their heart health while givingdoctors a reliable tool that saves time and improves patient care.
  • AI|ffinity — AI|ffinity provides NMR-AI drug discovery solutions: 4D-GraFID for protein structure determination up to 30 kDa, deepHitExplorer for fragment screening, and deepScaffOpt for lead optimization.
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Biotech Investor funding in Czech Republic at a glance

Stats across 30 deals from 24 investors.

24
investors
in Czech Republic Biotech startups
30
deals
in Czech Republic Biotech startups
$309k
median round
in Czech Republic Biotech startups
46%
lead investors
lead at least one round
37%
Seed
most common funding stage
92/8
VC / Angel
share of investor count

Access all 24 investors in Czech Republic Biotech startups.

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